Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response

Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to preci...

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Detalles Bibliográficos
Autores: Belhart, Keila, Sisti, Federico, Gestal, Mónica C., Fernández, Julieta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:Argentina
Institución:Universidad Nacional de La Plata
Repositorio:SEDICI (UNLP)
Idioma:inglés
OAI Identifier:oai:sedici.unlp.edu.ar:10915/160042
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/160042
Access Level:acceso abierto
Palabra clave:Biología
Inmunología
Microbiología
Biología molecular
patogénesis
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spelling Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune responseBelhart, KeilaSisti, FedericoGestal, Mónica C.Fernández, JulietaBiologíaInmunologíaMicrobiologíaBiología molecularpatogénesisBordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica.Instituto de Biotecnología y Biología Molecular2023-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160042enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2024-05-08T13:20:43Zoai:sedici.unlp.edu.ar:10915/160042Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292024-05-08 13:20:44.06SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
spellingShingle Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
Belhart, Keila
Biología
Inmunología
Microbiología
Biología molecular
patogénesis
title_short Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_fullStr Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full_unstemmed Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_sort Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
dc.creator.none.fl_str_mv Belhart, Keila
Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
author Belhart, Keila
author_facet Belhart, Keila
Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
author_role author
author2 Sisti, Federico
Gestal, Mónica C.
Fernández, Julieta
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Inmunología
Microbiología
Biología molecular
patogénesis
topic Biología
Inmunología
Microbiología
Biología molecular
patogénesis
description Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica.
publishDate 2023
dc.date.none.fl_str_mv 2023-05-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/160042
url http://sedici.unlp.edu.ar/handle/10915/160042
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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